Purification And Characterization Of Cellulobiase

Purification and characterization of Cellulobiase By John Bullock override In this experiment we determined the enzyme activity for cellulobiase. Two buffers were fasten in severalise to quantitate the proteins using ion exchange chromatography. Protein decision was so carried out using linear regression data. Once the protein decision was through we then selected the correct proteins to run our enzyme assay. When measuring the absorbance in this case, we were measuring the product that the enzyme produced. So, the higher the absorbance, the more products were produced, which means the enzyme was operating(prenominal) more rapidly. Based on our results Protein 1 Eluate 2 had the intimately enzyme activity for that protein and Protein 2 Eluate had the most enzyme activity overall. Introduction Cellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. Cellulose is a linear polysaccharide of glucose residues connected by ?-1 ,4 linkages. In nature cellulose is usually associated with varied polysaccharides such as xylan or lignin. It is the skeletal basis of phytology cell walls. Cellulose is the most abundant organic source of food, hassle and chemicals. However, its usefulness is enumerateent upon its hydrolysis to glucose.

Acid and high temperature degradation be unsatisfactory in that the resulting sugars are decomposed; also, waste cellulose contains impurities that generate unwanted by-products under these harsh conditions. Although cellulases are distributed throughout the biosphere, they are by and large manifested in fungi and microbial sources. It is possible to hall! ow these enzymes in order to study the enzymatic activity. This can be done in a stepwise manner. Initially there must be separation of the proteins. This is done using a vicissitude of chromatography here Ion Exchange chromatography was used. Ion exchange chromatography involves separation base on charge; the pI of the protein and the strength of the binding depend on the size...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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